Distinctive Effects of Cytochalasin B in Chick Primary Myoblasts and Fibroblasts
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منابع مشابه
Distinctive Effects of Cytochalasin B in Chick Primary Myoblasts and Fibroblasts
Actin-based structures play fundamental roles in cellular functions. However it remains controversial how cells cope with the absence of F-actin structures. This report focuses on short- and long-term effects of cytochalasin B (CB) on actin-complexes in fibroblasts and myoblasts. Thirty min of CB treatment dispersed subplasma actin cortices, lamellipodia, ruffled membranes, stress fibers and ad...
متن کاملThe use of cytochalasin B to distinguish myoblasts from fibroblasts in cultures of developing chick striated muscle.
Cytochalasin B (5 mug/ml) elicits a differential effect on myoblasts and fibroblasts in culture. After 1 day in culture in the presence of the drug, two types of cells were observed, round cells and cells with elongated arms, designated "arborized" cells. Both cell types were examined in the electron microscope. The round cells contained aggregates of thin and thick filaments as well as a few i...
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INTRODUCTION the addition of the drug . Concentrations of about 3 μg/ml were required to observe altered cell morphology. The possibility was considered that depolymerization of microfilaments-with a resulting change in cell shape and function--resulted from a rapid depletion of intracellular energy levels brought about by the inhibition of sugar transport . The effects of cytochalasin B and io...
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Six different proteins are found to be reproducibly exposed on the cell surface of chicken embryo fibroblasts (CEF) by the criterion of lactoperoxidase-catalyzed iodination (250,000, 185,000, 130,000, 100,000, 87,000, and 75,000 daltons). We wondered whether cell enucleation might lead to a differential partition of these surface proteins with the karyoplast or cytoplast membrane. We found that...
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although there are studies on pragmatic assessment, to date, literature has been almost silent about native and non-native english raters’ criteria for the assessment of efl learners’ pragmatic performance. focusing on this topic, this study pursued four purposes. the first one was to find criteria for rating the speech acts of apology and refusal in l2 by native and non-native english teachers...
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ژورنال
عنوان ژورنال: PLOS ONE
سال: 2016
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0154109